GSM1936774: NAMEC8 H3K9me2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me2
Cell type
Cell type Class
Breast
Cell type
HMLE
NA
NA
Attributes by original data submitter
Sample
source_name
Mesenchymal derivatives of HMLE cells
cell type
NAMEC8
chip antibody
H3K9me2 antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology; #9005); PHF2 Antibody (Cell Signaling Technology, Catalog #3497 Lot#2) To make libraries, TruSeq ChIP protocol was followed as described in the kit (Illumina; Catalog # IP-202-1012) manual Libraries were pooled together and sequenced on the HiSeq 2500 sequencer using the Standard sequencing protocols. Images analysis and base calling was done using the Standard Illumina pipeline, and then demultiplexed into fastq files