GSM1936758: IFN 24hrs HIRA Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
IMR90_IFN_24hrs
cell line
IMR90
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Native ChIP was performed for HIRA and HA-H3.3 as described previously (Rai et al. 2014). Antibodies used for ChIP were: Cocktail of mouse mAbs to HIRA (approximately equimolar mixture of WC15, WC19, WC117, WC 119 (Hall et al. 2001)), HA (Millipore 05904) (for anti-HA-H3.3 ChIP). Mouse mAb to HA tag (Covance, MMS-101R) was used as species/class-matched negative control Abs. ChIP libraries were prepared for sequencing using standard illumina protocols