Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Myod1

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
C2C12
biomaterial_provider
Barbara Wold lab
lab
Caltech-m
lab description
Wold - Califonia Institute of Technology
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
C2C12
cell organism
mouse
cell description
Myoblast cell line derived from thigh muscle of C3H mice after crush injury
cell sex
F
antibody
MyoD_(sc-32758)
antibody description
mouse monoclonal antibody raised against recombinant MyoD of mouse origin with an epitope mapping to amino acids 180-189. Antibody Target: MyoD
antibody targetdescription
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-32758
control
Control_32bp
control description
This data represents a control being compared to experiments using read length of 32 bp
treatment
None
treatment description
No special treatment or protocol applies
age
immortalized
age description
Immortal cells
control
Control_32bp
control description
This data represents a control being compared to experiments using read length of 32 bp
controlid
10135
labexpid
10333
labversion
Myoblast
replicate
1
strain
C3H
strain description
Strain C3H, Very related to C57BL/6

Sequenced DNA Library

library_name
GSM915186: Caltech_ChipSeq_C2C12_MyoD_(sc-32758)_Control_32bp
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
35159837
Reads aligned (%)
59.5
Duplicates removed (%)
7.3
Number of peaks
19746 (qval < 1E-05)

mm9

Number of total reads
35159837
Reads aligned (%)
59.5
Duplicates removed (%)
7.4
Number of peaks
19806 (qval < 1E-05)

Base call quality data from DBCLS SRA