MATa his3delta1 leu2delta met15delta ura3delta Ptet-SNF2 gcn5delta::kanMX4 ydj1delta::hphMX4
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
In ChIP-seq experiments, cells were cross-linked with formaldehyde before harvesting. In MNase-seq experiments, nuclei were prepared from unfixed yeast cells and then digested with MNase. Mono-nucleosomal DNA was gel-purified (~150 bp). See paper for details. The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.