GSM5903236: H1963 ASXL3 sgMBD6 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ASXL3
Cell type
Cell type Class
Lung
Cell type
NCI-H1963
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Small Cell
Attributes by original data submitter
Sample
source_name
NCI-H1963
cell type
small cell lung cancer
passages
Low passages (6-10)
cell line
H1963_ASXL3_sgMBD6_1
chip antibody
anti-ASXL3 (home made)
genotype
MBD6 depleted cells
Sequenced DNA Library
library_name
GSM5903236
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using QIAGEN RNeasy kit. DNA after ChIP protocol was isolated using QIAGEN PCR purification kit. ChIP-sequencing libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250- 450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit. RNA-sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA Preparation Kit with Ribo-Depletion. Input RNA quality was validated using the Agilent RNA 6000 Nano Kit. 200ng-1 µg of total RNA was used as starting material. Libraries were validated using the Agilent DNA 1000 Kit