GSM1924875: Sox13hi and Sox13lo Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Gamma delta T cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse thymocytes
strain
C57BL/6
genotype
TCRbeta KO.Sox13 ECFP KI/+
cell type
sorted gd T thymocytes
index
ATCACG
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1 million cells for SOX13 ChIPseq and 0.25 milion cells for H3K4me3 and H3K27me3 were sorted (gd+, CD24+, CFPhi or CFPlo/-), crosslinked with 1% formaldehyde, sonicated, and then subjected to chromatin immunoprecipitation with anti-SOX13, anti-H3K4me3 and anti-H3K27me3 antibodies. 300-500 bp DNA fragments were obtained after reverse-cross linking. ChIP DNA fragments were processed for ChIPseq with Illumina adapters and Illumina primers (Primer1.1 and Primer2.1). After adapter ligation, DNAs were PCR-amplified for 17 cycles and library fragments of 300-500 bp were extracted from an agarose gel.