GSM5866832: D28 WT KIDNEY ORGANOID HNF1A CHIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
HNF1A
Cell type
Cell type Class
Kidney
Cell type
Kidney organoids
NA
NA
Attributes by original data submitter
Sample
source_name
Kidney organoid cells at D28 of renal differentiation
cell type
WT-hPSC (H9) derived kidney organoid cells
chip antibody
Rb anti-HNF1A (ab96777)
age
Day 28 of renal differentiation
Sequenced DNA Library
library_name
GSM5866832
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were clarified from sonicated kidney organoids following dissociation using TrypLE. Immunoprecipitation of HNF1A protein was performed using HNF1A antibody (ab96777). Library preparation was done using a commercially available kit, NEB# E7645 NEBNext Ultra II DNA Library Prep Kit for Illumina KIT® following the manufacturer's protocol. During the library construction, the ChIP-ed DNA underwent End Repair, Adapter ligation, size selection (for ~150bp insert) and 16 cycles PCR enrichment to generate the final sequencing ready library. The quality of the library was checked using Agilent D1000 ScreenTape. The final library should have a fragment size of ~270bp insert plus adaptor and PCR primer sequences). The quality of the library was checked using Agilent D1000 ScreenTape. A single peak was expected and was observed indicating that the library was good and suitable for sequencing.