Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Gonad

Cell type

Oocytes

MeSH Description

Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Sample

source_name

Oocyte

background strain

CD1

antibody

Rabbit IgG, Abcam ab1791

developmental stage

Oocyte nuclei (p10)

genotype

Wild-type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq postnatal day 10 oocyte nuclei were obtained through enzymatic digestion of CD1 ovaries, nuclei extraction, and subsequent FACS sorting. ChIP lysis and immunoprecipitation were performed using the True MicroChIP kit (Diagenode AB-002- 0016), with modifications. ChIP-Seq libraries were generated from immunoprecipitated DNA using the NEBNext Ultra End Repair/dA-Tailing Module (E7742) followed by adaptor ligation, DNA purification and library amplification.

Sequencing Platform

instrument_model

Illumina HiSeq 1000

mm10

Number of total reads

34044890

Reads aligned (%)

91.7

Duplicates removed (%)

83.6

Number of peaks

121 (qval < 1E-05)

mm9

Number of total reads

34044890

Reads aligned (%)

91.6

Duplicates removed (%)

83.4

Number of peaks

97 (qval < 1E-05)