Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
Pol2(phosphoS2)
antibody antibodydescription
Rabbit polyclonal against peptide conjugated to KLH derived from within residues 1600 - 1700 of S. cerevisiae RNA polymerase II CTD repeat YSPTSPS, phosphorylated at S2. Antibody Target: POL2S2
antibody targetdescription
RNA polymerase II, large subunit- specific for phosphorylated C-terminal domain.
antibody vendorname
Abcam
antibody vendorid
ab5095
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
age
immortalized
age description
Immortal cells
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
CH12/None/Input/IgG-rab
replicate
1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM912931: Stanford_ChipSeq_CH12_Pol2(phosphoS2)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
51620997
Reads aligned (%)
93.8
Duplicates removed (%)
9.8
Number of peaks
19366 (qval < 1E-05)

mm9

Number of total reads
51620997
Reads aligned (%)
93.6
Duplicates removed (%)
9.8
Number of peaks
19358 (qval < 1E-05)

Base call quality data from DBCLS SRA