Antigen

Antigen Class

TFs and others

Antigen

JUNB

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS cells

cell line

U2OS-derived UTA6 cells

cell type

osteosarcoma cell

clone

U2OS-derived UTA6 clone

chip antibody

JUNB (Cell Signaling (#3753))

treatment

untreated

Sequenced DNA Library

library_name

GSM5857627

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and JUNB complexes were isolated with the appropriate antibodies. ChIP-seq libraries were prepared using the TruSeq ChIP sample reparation kit from Illumina by the MGX sequencing Platform (Montpellier, France) ChIP-seq - Instrument model : Illumina HiSeq 2500 (MGX Platform, Montpellier France).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

52484169

Reads aligned (%)

96.5

Duplicates removed (%)

7.1

Number of peaks

9794 (qval < 1E-05)

hg19

Number of total reads

52484169

Reads aligned (%)

95.7

Duplicates removed (%)

8.6

Number of peaks

9881 (qval < 1E-05)