GSM5840551: CTCF 16HD rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC RUES1
NA
NA
Attributes by original data submitter
Sample
source_name
human embryonic stem cells
cell line
RUES1
chip antibody
CTCF Active Motif (#61311, Lot 34614003)
treatment
7% 1,6-Hexanediol for 5 minutes, wash, incubate with fresh media for 5 minutes at 37 deg C.
Sequenced DNA Library
library_name
GSM5840551
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Briefly, approximately 10 million cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10 min and neutralized with 0.125M glycine. After sonication, ~50µg soluble chromatin was incubated with 1-5μg of antibody at 40C overnight. Immunoprecipitated complexes were collected using Dynabeads A/G (Invitrogen). Subsequently, immuno-complexes were washed, DNA extracted, and purified by QIAquick Spin columns (Qiagen). For ChIP-seq, the extracted DNA was ligated to Illumina UDI adaptors followed by deep sequencing with Illumina's HiSeq 4000 according to the manufacturer's instructions. ChIP enriched DNA was purified, and 10 ng used to prepare sequencing libraries with the KAPA HTP/LTP Library Preparation Kits