GSM5838064: ISL1 WT d18MNP Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ISL1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
Motor neuron progenitor cells
cell type
d18 hiPSC-derived motor neuron progenitor cells (d18MNP)
genotype
WTC11
ChIP
ChIP with anti-human-ISLET-1 antibody (Cat. AF1837, R&D Systems)
treatment
untreated
molecule subtype
TF-bound DNA
molecule
genomic DNA
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM5838064
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
In brief, progenitor cells were collected from plates and fixed and frozen. Thawed cells were then subjected to a nuclear extraction protocol involving shearing of chromatin. They rotated O/N with primary antibody (input saved prior) then antibody-TF complexes were captured via protein G Dynabeads. After washes, sample was eluted and then treated with proteinase and RNAse, leaving DNA to be further processed for ChIP-seq. In brief, samples were prepared for library sequencing using the NEBNExt Ultra II DNA Library Kit for Illumina, and following manufacturer instructions. Sample integrity was confirmed via Bioanalyzer (Agilent) and then samples were run on an Illumina NextSeq 500 or an Illumina HiSeq 4000.