Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Placenta
Cell type
Differentiated trophoblast stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Differentiated trophoblast stem cells
cell type
Day 2 differentiated trophoblast stem cells
strain
C57Bl6/J; 129/SvEv

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were precleared with Protein G Dynabeads. Complexes were then isolated with 7µg anti-Blimp1 monoclonal antibody (Novus, clone 3H2-E8) linked to Protein G Dynabeads. Sequencing libraries were prepared using the Illumina ChIP-seq DNA Sample Prep Kit. Libraries were size selected at ~200bp and four libraries were multiplexed per lane on a HiSeq 2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
29887669
Reads aligned (%)
96.3
Duplicates removed (%)
13.2
Number of peaks
813 (qval < 1E-05)

mm9

Number of total reads
29887669
Reads aligned (%)
96.2
Duplicates removed (%)
13.7
Number of peaks
792 (qval < 1E-05)

Base call quality data from DBCLS SRA