Antigen

Antigen Class

TFs and others

Antigen

HSF1

Cell type

Cell type Class

Prostate

Cell type

PC-3M

NA

NA

Sample

source_name

cancer cell line

cell line

PC3M X1 Clone

vector

N/A

sirna/sgrna

sgHSF2.1

antibody

HSF1 (H311)

Sequenced DNA Library

library_name

GSM5828091

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

KAPA HTP Library Preparation Kit RNA was extracted using the Qiagen RNeasy kit using the Qiashredder for homogenization of cells grown in vitro and a rotor-stator homogenizer for xenograft tumors

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

4455374

Reads aligned (%)

71.4

Duplicates removed (%)

8.6

Number of peaks

4016 (qval < 1E-05)

hg19

Number of total reads

4455374

Reads aligned (%)

71.0

Duplicates removed (%)

8.9

Number of peaks

3996 (qval < 1E-05)