HCT116 colon cancer cells, p53-WT, camptothecin, p53 ChIP
cell line
HCT116
cell type
colon cancer cell line
genotype/variation
p53-WT
treatment
camptothecin
chip antibody
p53
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared by sonication using Bioruptor to an average fragment size of 500 bp, immunoprecipitated using the indicated antibody, purified and sequenced using Illumina HiScanSQ following protocols recommended by the manufacturer. Libraries were prepared according to Illumina's instructions.