Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

SKM1

experiment

Pfeiffer_Skm1_Toledo/Exp016-3

histone modification

H3

mnase units

150u

treatment

DMSO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

250362

Reads aligned (%)

95.8

Duplicates removed (%)

3.2

Number of peaks

1 (qval < 1E-05)

hg19

Number of total reads

250362

Reads aligned (%)

95.0

Duplicates removed (%)

3.3

Number of peaks

4 (qval < 1E-05)