Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

experiment

Low_input_K562/Exp015-2

histone modification

H3K27me3

cell number

5000c

mnase units

15u

treatment

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

3631951

Reads aligned (%)

91.9

Duplicates removed (%)

1.8

Number of peaks

31 (qval < 1E-05)

hg19

Number of total reads

3631951

Reads aligned (%)

91.4

Duplicates removed (%)

1.9

Number of peaks

33 (qval < 1E-05)