Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

experiment

C646_GSK126/Exp004-9

histone modification

H3K27ac

mnase units

25u

treatment

DMSO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

347498

Reads aligned (%)

96.2

Duplicates removed (%)

3.3

Number of peaks

19 (qval < 1E-05)

hg19

Number of total reads

347498

Reads aligned (%)

95.9

Duplicates removed (%)

3.3

Number of peaks

18 (qval < 1E-05)