GSM5825396: AC16 cell line-R2 ATF3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ATF3
Cell type
Cell type Class
Cardiovascular
Cell type
AC16
NA
NA
Attributes by original data submitter
Sample
source_name
AC16 cardiomyocyte cell line
cell type
human cardiomyocyte cell line
treatment
hypoxia 3h and reoxygenation 3h
chip antibody
ATF3(CST, #33593)
Sequenced DNA Library
library_name
GSM5825396
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. After the DNA sample passes the test, it is randomly interrupted by covaris ultrasonic crusher, and then repaired at the end, added with a tail and added with a tail，The whole library was prepared by sequencing, purification and PCR amplification. After the library is built, qubit2.0 is used first perform preliminary quantification, dilute the library to 1.5ng/ul, and then use Agilent 2100 to detect the insert size of the library. After the size meets the expectation, the effective concentration of the library is accurately quantified by Q-PCR (the effective concentration of the library is > 2nm), to ensure the quality of the library.