GSM5788899: Ctr1 LCL ATRX Abcam; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ATRX
Cell type
Cell type Class
Blood
Cell type
Lymphoblastoid cell line
Tissue
blood
Lineage
mesoderm
Description
parental cell type to lymphoblastoid cell lines
Attributes by original data submitter
Sample
source_name
human lymphoblastoid cell line
phenotype
unaffected
antibody
Abcam ab97508
crosslinking
two steps cross-linking
Sequenced DNA Library
library_name
GSM5788899
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
108 cells were crosslinked with 2 mM EGS for 45 minuntes and 1% FA for 20 minutes (ATRX) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (Runx3). Cells were lysed and sonicated on the Covaris to give fragments of 100-300bp. Immunoprecipitation was performed by incubation of diluted chromatin overnight. NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs).