Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Neural

Cell type

Retina

MeSH Description

The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

Sample

source_name

retina_H3K27Ac_P21

tissue

retina

genotype

wildtype

antibody

H3K27Ac-AB2

age

P21

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

MinElute PCR-purification kit (QIAGEN) ChIP was performed using the iDeal ChIP-seq kit (Diagenode).

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

28556213

Reads aligned (%)

100.0

Duplicates removed (%)

2.8

Number of peaks

6699 (qval < 1E-05)

mm9

Number of total reads

28556213

Reads aligned (%)

99.1

Duplicates removed (%)

5.7

Number of peaks

15630 (qval < 1E-05)