Antigen

Antigen Class

TFs and others

Antigen

POU2F1

Cell type

Cell type Class

Prostate

Cell type

Prostatic Neoplasms, Castration-Resistant

MeSH Description

Tumors or cancer of the PROSTATE which can grow in the presence of low or residual amount of androgen hormones such as TESTOSTERONE.

Sample

source_name

AR-positive CRPC cells

cell type

AR-positive CRPC cells

culture

2D cultured from PDX

treatment

DHT 10 nM for 24 h + PI for 48 h

chip antibody

OCT1 (abcam 15112)

Sequenced DNA Library

library_name

GSM5772720

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. The Chromatin immunoprecipitated DNA fragments (approx.10 ng in 30 µl water) are end repaired by using T4 DNA polymerase, Klenow DNA polymerase and T4 polynucleotide kinase in the presence of dNTPs. And an 'A' base is added to the 3'end of the blunt phosphorylated DNA fragments, using the polymerase activity of Klenow fragment. Then, adaptors (Adaptor oligo mix, Illumina) are ligated to the ends of the DNA fragments. The resulting DNA fragments are purified by MinElute column (QIAGEN). 300 ~ 500 bp DNA fragments are purified by using E-Gel SizeSelect agarose Gels (Invitrogen) according to manufacturer's instruction. Size selected adaptor-modified DNA fragments are amplifiedand the resulting PCR products are purified by MinElute column (QIAGEN). The size and amount of DNA fragments (libraries) are validated by Bioanalyzer (Agilent).

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

30367426

Reads aligned (%)

4.4

Duplicates removed (%)

32.0

Number of peaks

161 (qval < 1E-05)

hg19

Number of total reads

30367426

Reads aligned (%)

4.4

Duplicates removed (%)

32.4

Number of peaks

166 (qval < 1E-05)