Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Adipocyte

Cell type

Adipose stem cells

NA

NA

Sample

source_name

subcutaneous abdominal ASC

cell type

hASCs

method

ChIP-seq

antibody

CTCF

Sequenced DNA Library

library_name

GSM5764506

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fat biospsies were collected and SVF were isolated by centrifugation. For ATAC-seq, nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina).DNA was purified with MinElute PCR Purification Kit (Qiagen). For ChIP-seq, Lysates were clarified from sonicated nuclei and histone/CTCF or RNAPII-DNA complexes were isolated with antibody. ATAC-seq, ChIP-seq and ChIRP-seq libraries were prepared and sequenced using standard Illumina protocols for HiSeq 2500 instrument. ATAC-seq, ChIP-seq, ChIRP-seq

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

28723932

Reads aligned (%)

97.8

Duplicates removed (%)

27.4

Number of peaks

56000 (qval < 1E-05)

hg19

Number of total reads

28723932

Reads aligned (%)

97.6

Duplicates removed (%)

27.6

Number of peaks

55817 (qval < 1E-05)