GSM5733008: PDX2 ChIP STAT5B sgNS IP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
STAT5B
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
PDX2
cell type
PDX2
treatment
sgNS
antibody
STAT5B (Ab178941, Abcam)
Sequenced DNA Library
library_name
GSM5733008
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Active Motif ChIP kit ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (Kapa Biosystems, Wilmington, NC) following manufacturer's protocol. ChIP DNA was subjected to end repair with subsequent 3' adenylation to create 3' dA overhang suitable for adaptor ligation. Illumina adaptors were ligated to both ends of the DNA and amplified using 10 cycles of PCR with primers specific to the adaptor sequences to generate amplicons of approximately 200–500 bp in size. Libraries were purified using KAPA Pure Beads (Kapa Biosystems). Each library was quantified by Qubit fluorometer (Thermo Fisher Scientific) and evaluated by the 2100 Bioanalyzer (Agilent Technologies). The libraries were amplified using the cBot (Illumina) and single end reads with 50-nt length were obtained using Illumina HiSeq2500 instrument.