Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Leukemia, Lymphoid

MeSH Description

Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.

Sample

source_name

MLL-ENL leukemia

histone

H3K4Me3

genetic modification

ΔERT2 + Hhex-F

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was fragmented in a Covaris Sonicator (30 minutes, 4°C) then protein-DNA complexes immunoprecipitated from clarified chromatin fractions using H3K27Me3 (2μg; Millipore: 07-449) or H3K4Me3 (2μg; Millipore: 07-473) antibodies and 30μl of Protein A-sepharose. For Hhex-Flag ChIP, 30μl of anti-Flag (M2)-agarose beads (Sigma) was added directly to clarified chromatin fractions. Following reversal of crosslinks and RNaseI and Proteinase K treatments, extracted DNA was purified using the QIAquick purification kit. ChIP-Seq DNA libraries were constructed using TruSeq Nano DNA sample preparation according to the manufacturer’s instructions.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

113161798

Reads aligned (%)

87.8

Duplicates removed (%)

90.2

Number of peaks

21288 (qval < 1E-05)

mm9

Number of total reads

113161798

Reads aligned (%)

87.7

Duplicates removed (%)

90.2

Number of peaks

21301 (qval < 1E-05)