Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

LNCaP MYC cells

cell line

c-Myc overexpressing LNCaP

antibody

anti-H3K27me3, diagenode (pAb-195-050)

agent

R1881+Doxycycline

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies, see study for more information. Libraries were prepared according to published instructions for the ChIP-exo libraries (AR/MYC, see study for details) and Illumina's instructions accompanying the TruSeq library preparation kit for the histone ChIP-seqs.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

40966961

Reads aligned (%)

99.4

Duplicates removed (%)

5.2

Number of peaks

910 (qval < 1E-05)

hg19

Number of total reads

40966961

Reads aligned (%)

98.8

Duplicates removed (%)

6.0

Number of peaks

902 (qval < 1E-05)