Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP MYC cells
cell line
c-Myc overexpressing LNCaP
antibody
anti-MYC, RnD (AF3696)
agent
R1881

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies, see study for more information. Libraries were prepared according to published instructions for the ChIP-exo libraries (AR/MYC, see study for details) and Illumina's instructions accompanying the TruSeq library preparation kit for the histone ChIP-seqs.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21590129
Reads aligned (%)
60.1
Duplicates removed (%)
38.3
Number of peaks
3479 (qval < 1E-05)

hg38

Number of total reads
21590129
Reads aligned (%)
62.0
Duplicates removed (%)
37.0
Number of peaks
3578 (qval < 1E-05)

Base call quality data from DBCLS SRA