GSM1906346: ChIP-seq Rpc4 IMR90hTert cells, (FBS) rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase III
Cell type
Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
immortalized fetal lung fibroblasts
experiment
ChIP-seq
cell line
IMR90hTert
passage
6-13
treatment
Cells grown in fetal bovine serum
chip antibody
anti-Rpc4 (Canella et al, 2010; homemade antibody raised in rabbit against peptide CSPDFESLLDHKHR of the RNA polymerase III subunit POLR3D (Rpc4))
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChiP-seq was performed as described (Canella et al., 2010) Libraries were prepared with 12 ng of ChIP DNA (ChIP-seq) or genomic DNA (INPUT) according to manufacturer's's instruction with the ChIP-seq DNA Sample prep kit” (Illumina, 1003473)