Antigen

Antigen Class

TFs and others

Antigen

ZEB1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

breast; derived from metastatic site: pleural effusion

tissue

breast

cell type

Breast carcinoma cell line

cell line

MCF7-V

treatment

2 µg/ml doxycyline

genotype

ZEB1 expression

chip antibody

ZEB1, Proteintech, catalog 21544-1-AP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Immunoprecipitated DNA was extracted using the phenol–chloroform–isoamyl alcohol method. Library was prepared starting with 5 ng of ChIP-enriched DNA as starting material and processed with the Illumina TruSeq ChIP kit according to manufacturer specifications

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

44765409

Reads aligned (%)

94.8

Duplicates removed (%)

26.1

Number of peaks

23224 (qval < 1E-05)

hg19

Number of total reads

44765409

Reads aligned (%)

94.2

Duplicates removed (%)

26.3

Number of peaks

22666 (qval < 1E-05)