ChIP-Atlas: SRX13234467

Antigen

library_name: GSM5704555
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Cells were transduced overnight twice in consecutive days and media replaced in between. After the second transduction, culture media was replaced by normal growth media and this was considered day 0. Cells were collected 2 days after viral transduction Cultured cells were fixed in 11 % formaldehyde for 15 min at room temperature on a rotating platform. The formaldehyde was quenched by adding 125 nM glycine. The chromatin was sheared and immunoprecipitated with the antibody of interest. A control sample consisting of sonicated chromatin that was not immunoprecipitated was also sequenced. Illumina sequencing libraries were prepared from ChIP and Input DNAs by standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single-end).