Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cardiovascular

Cell type

Heart

MeSH Description

The hollow, muscular organ that maintains the circulation of the blood.

Sample

source_name

input DNA

strain

C57BL/6

genotype/variation

KLF15 knockout

tissue

heart

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Hearts were harvest at ZT10, 3 hearts were pooled for chromatin extraction. Libraries were prepared according to Illumina's instructions accompanying the Truseq ChIPseq Sample Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

100632242

Reads aligned (%)

95.7

Duplicates removed (%)

12.8

Number of peaks

676 (qval < 1E-05)

mm9

Number of total reads

100632242

Reads aligned (%)

95.6

Duplicates removed (%)

12.8

Number of peaks

705 (qval < 1E-05)