GSM1893972: ChIP-Seq analysis of H3K36me3 in mouse ESCs under shControl knockdown treatment replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K36me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESC shControl WITH LIF
cell type
mouse embryonic stem cells
strain
C57BL/6 X 129/Sv
cell line
KH2
chip antibody
H3K36me3 (Abcam, ab9050)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million cells were treated withmicrococcal nuclease to generate mono-nucleosomes and for the ChIP chromatin was precipitated using 10 micrograms of antibody against H3K4me3 (Millipore, 07-473), H3K79me2 (Active Motif, 39143) and H3K36me3 (Abcam, ab9050). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.