GSM1893999: ChIP-Seq analysis of Ser2-Phosphorylated PolII in mouse ESCs under shControl knockdown treatment replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESC shControl WITH LIF
cell type
mouse embryonic stem cells
strain
C57BL/6 X 129/Sv
cell line
KH2
chip antibody
Ser-2-Phosphorylated RNA polymerase II: Active Motif, clone 3E10
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million cells were used for the ChIP and precipitated using 10 micrograms of antibody against Ser-5-Phosphoryalted RNA polymerase II (Abcam, ab5131 ) and against Ser-2-Phosphoryalted RNA polymerase II (Active Motif, clone 3E10) Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.