GSM1894008: ChIP-Seq analysis of Paf1 in mouse ESCs under shControl knockdown treatment replicate 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Paf1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESC shControl WITH LIF
cell type
mouse embryonic stem cells
strain
C57BL/6 X 129/Sv
cell line
KH2
chip antibody
Paf1 antibody, serum purified
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million cells were used for the ChIP and precipitated using 2 micrograms of serum-purified antibody against Paf1. Original reference: Zhu B. et al. (2005) "The human PAF complex coordinates transcription with events downstream of RNA synthesis" Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.