Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Embryonic fibroblast

Cell type

3T3-L1

Tissue

Embryo

Cell Type

Fibroblast

Sample

source_name

3T3-L1 adipocytes

cell type

3T3-L1 adipocytes

ChIP

PolII

day

Day 6

sirna/shrna

-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclear fraction was incubated with each antibody and histone-DNA or SETDB1-DNA complexes were isolated. Sonicated DNA was incubated with MBD domain of MBD1 and m5CpG was isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

26277739

Reads aligned (%)

97.3

Duplicates removed (%)

32.6

Number of peaks

4341 (qval < 1E-05)

mm9

Number of total reads

26277739

Reads aligned (%)

97.0

Duplicates removed (%)

32.7

Number of peaks

4379 (qval < 1E-05)