Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Embryonic fibroblast

Cell type

3T3-L1

Tissue

Embryo

Cell Type

Fibroblast

Sample

source_name

3T3-L1 preadipocytes

cell type

3T3-L1 preadipocytes

ChIP

H3K9me3

day

Day 0

sirna/shrna

-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclear fraction was incubated with each antibody and histone-DNA or SETDB1-DNA complexes were isolated. Sonicated DNA was incubated with MBD domain of MBD1 and m5CpG was isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

39731377

Reads aligned (%)

82.7

Duplicates removed (%)

39.0

Number of peaks

931 (qval < 1E-05)

mm9

Number of total reads

39731377

Reads aligned (%)

82.3

Duplicates removed (%)

38.9

Number of peaks

1049 (qval < 1E-05)