Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryonic fibroblast

Cell type

C3H/10T1/2

NA

NA

Sample

source_name

10T1/2 MSCs

cell type

10T1/2 mesenchymal stem cells

ChIP

H3K4me3

day

-

sirna/shrna

-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclear fraction was incubated with each antibody and histone-DNA or SETDB1-DNA complexes were isolated. Sonicated DNA was incubated with MBD domain of MBD1 and m5CpG was isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

20073218

Reads aligned (%)

98.5

Duplicates removed (%)

32.0

Number of peaks

21588 (qval < 1E-05)

mm9

Number of total reads

20073218

Reads aligned (%)

98.3

Duplicates removed (%)

32.1

Number of peaks

21653 (qval < 1E-05)