Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

TT2 ESCs

cell type

TT2 embryonic stem cells

ChIP

H3K27me3

day

-

sirna/shrna

-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclear fraction was incubated with each antibody and histone-DNA or SETDB1-DNA complexes were isolated. Sonicated DNA was incubated with MBD domain of MBD1 and m5CpG was isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

38056453

Reads aligned (%)

98.3

Duplicates removed (%)

11.3

Number of peaks

2193 (qval < 1E-05)

mm9

Number of total reads

38056453

Reads aligned (%)

98.2

Duplicates removed (%)

11.4

Number of peaks

2256 (qval < 1E-05)