Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7-xenograft

tissue

Snap-frozen tissue

antibody

H3K4me2 (Millipore, 07-030)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIPseq:ThruPLEX-FD kits (Rubicon Genomics) was used and 75 bp single-end reads were sequenced on a NextSeq 500 instrument (Illumina). For RNAseq: TruSeq RNA Sample Preparation Kit (Illumina) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

55299015

Reads aligned (%)

80.9

Duplicates removed (%)

2.0

Number of peaks

86023 (qval < 1E-05)

hg19

Number of total reads

55299015

Reads aligned (%)

80.7

Duplicates removed (%)

2.1

Number of peaks

85768 (qval < 1E-05)