Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Digestive tract

Cell type

Colorectal carcinoma

NA

NA

Sample

source_name

Primary colorectal carcinoma

tissue

FFPE

antibody

H3K36me3 (Abcam ab9050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIPseq:ThruPLEX-FD kits (Rubicon Genomics) was used and 75 bp single-end reads were sequenced on a NextSeq 500 instrument (Illumina). For RNAseq: TruSeq RNA Sample Preparation Kit (Illumina) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

38709533

Reads aligned (%)

95.1

Duplicates removed (%)

9.6

Number of peaks

1268 (qval < 1E-05)

hg19

Number of total reads

38709533

Reads aligned (%)

95.0

Duplicates removed (%)

9.7

Number of peaks

1367 (qval < 1E-05)