Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Embryonic heart

NA

NA

Sample

source_name

heart

developmental stage

E10.5

strain

Hand2 3xFlag mice (Osterwalder et al., Dev Cell, 2014)

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

A modified ChIP protocol was used based on the protocols described in Kim et al. 2007 (PMID: 18265397) and Visel et al. 2009 (PMID: 19212405) DNA library Prep Kit from New England Biolabs

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

60489198

Reads aligned (%)

96.9

Duplicates removed (%)

26.6

Number of peaks

15191 (qval < 1E-05)

mm9

Number of total reads

60489198

Reads aligned (%)

96.7

Duplicates removed (%)

26.6

Number of peaks

14988 (qval < 1E-05)