GSM5651834: p53, DAXX WT cells; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP53
Cell type
Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma
Attributes by original data submitter
Sample
source_name
U87 glioblastoma cells
cell line
U87 glioblastoma cells
ChIP
p53
daxx status
WT
Sequenced DNA Library
library_name
GSM5651834
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Etoposide (10 uM) treated cells were harvested after 24 hours of treatment, fixed with formaldehyde for 10 min followed by quenching with glycine. 25 million cells per sample were sonicated using Covaris sonicator to obtain fragments of size between 100-500 bp. Chromatin was incubated with 10 mg of p53 antibody (Santa Cruz sc-126X) overnight followed by pull-down using protein- G Dynabeads (life technologies). Beads were washed, eluted and reverse cross-linked overnight. IP DNA was purified using Sigma PCR purification kit. Libraries were prepared using Illumina protocol and sequencing 75bp single end on Illumina Nextseq500 on high-output mode