GSM5625651: sgCXCR4 BRD4 ChIP Rep3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
cells
cell line
HCT116
condition
sgCXCR4
antibody
BRD4
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin bound to BRD4 was immunoprecipitated from fixed and sheared nuclei, with or without CEM207 treatment, in HCT116 cells bearing sgNT or sgCXCR4 (promoter). Enriched DNA from ChIP was barcoded and libraries were amplified, followed by size selection (fragment ranges between 250 and 1,000 bp), using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (E7103S) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (E6440S). Libraries were sequenced to a depth of 30 million base pairs (75-bp single-end reads) on an Illumina NextSeq 500.