Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6J

gender

male

chip antibody

H3K27me3 (07-449, Millipore)

treatment

sham operated

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from ~10 ng each of ChIP and input DNA with the use of an Ovation Ultralow DR Multiplex System (NuGEN, Cat No. 0330-32, 0331-32). Two rapid mode flow cells of an Illumina HiSeq 2500 were used for this analysis. Libraries were clonally amplified in the flow cells and sequenced with the use of Hiseq Control Software ver. 2.2.38 (Illumina) and a 51- nucleotide paired-end sequence. Image analysis and base calling were performed using real-time analysis software (RTA ver. 1.18.61, Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

90492082

Reads aligned (%)

98.8

Duplicates removed (%)

15.9

Number of peaks

295 (qval < 1E-05)

mm9

Number of total reads

90492082

Reads aligned (%)

98.6

Duplicates removed (%)

16.5

Number of peaks

306 (qval < 1E-05)