Immortalized Mouse Embryonic Fibroblast (MEF) cells in which both Mll2 alleles had been targeted by loxP (F/F cells)
treatment
24 hours exposure to tamoxifen
resulting cell type
MLL2 FC/FC MEF cells
chip antibody
anti-H2AX (Abcam, catalog# ab11175)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde. Nuclei were extracted in low salt conditions and burst in high salt before shearing the DNA to reduce the size to ~400 bp and performing ChIP with the appropriate antibodies. DNA libraries were prepared according to Illumina's standard instructions.