Antigen

Antigen Class

TFs and others

Antigen

LMNB1

Cell type

Cell type Class

Adipocyte

Cell type

Adipose stem cells

NA

NA

Sample

source_name

Human primary adipose stem cells

time point

Day -2 (proliferative; "Pro")

cell type

Proliferating adipose stem cells (ASCs) in growth medium

chip antibody

anti-lamin B1 antibody (Abcam ab16048), at 10 microgram per 10e7 cells

replicate

Rep1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP, cells were sonicated to produce chromatin fragments of ~200 base-pairs, lysates were clarified by sedimentation and histone-DNA or Lamin-DNA complexes were isolated from supernatants by ChIP using specific antibodies. RNA was purified from cell lysates using the RNEasy mini kit (Qiagen). ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a Nextseq500 or Novaseq (ChIP-seq) or on a Nextseq500 (RNA-seq) at the Norwegian Sequencing Center.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

68987231

Reads aligned (%)

88.0

Duplicates removed (%)

31.4

Number of peaks

2220 (qval < 1E-05)

hg19

Number of total reads

68987231

Reads aligned (%)

87.3

Duplicates removed (%)

33.3

Number of peaks

1792 (qval < 1E-05)