ChIP-Atlas: SRX1224468

Antigen

Cell type Class: Blood
Cell type: Lymphocytes
MeSH Description: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Libraries were prepared according to Illumina's instructions accompanying the ? Kit (#). Briefly, ChIP DNA was end repaired (End-it DNA Repair kit, Epicentre) and A-tailed (Klenow Exo-minus; Epicentre). Adaptors (Genomic Adaptor Oligo Mix; Illumina, San Diego, CA) were ligated to the ChIP-DNA (Fast-Link kit, Epicentre) for PCR amplification and PCR products were gel extracted. Here, the region of 200 – 300 bps was cut out of the gel, containing the expected DNA fragment, consisting of the 150 bp mononucleosomal ChIP-DNA plus 100 bp adaptors. Between each step the samples were cleaned with column based PCR purification kits (Qiagen). The prepared DNA was run on bioanalyzer to determine concentration and size distribution of the library. Libraries were sequenced with the Genome Analyzer II (Illumina). All sequencing libraries contained single-end 36-bp raw reads were mapped to the human genome (UCSC version hg19) using Bowtie (version 0.12.8).