Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFKB1

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD3/CD28 stimulated CD4+ T cells
cell type
CD4+ T cells
case or control
Control
age
10
Sex
male
ancestry
Caucasian
chip antibody
NFKB1 (Cell Signaling, catalog number 12540S, lot number 2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked and nuclei were sonicated as described previously (Lu et al. 2015). Libraries were prepared via ChIPmentation (Schmidl et al. 2015).

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
216265756
Reads aligned (%)
64.6
Duplicates removed (%)
88.0
Number of peaks
1298 (qval < 1E-05)

hg19

Number of total reads
216265756
Reads aligned (%)
63.9
Duplicates removed (%)
89.2
Number of peaks
886 (qval < 1E-05)

Base call quality data from DBCLS SRA