Antigen

Antigen Class

TFs and others

Antigen

NFKB1

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

CD3/CD28 stimulated CD4+ T cells

cell type

CD4+ T cells

case or control

Atopic Dermatitis case

age

12

Sex

female

ancestry

African American

chip antibody

NFKB1 (Cell Signaling, catalog number 12540S, lot number 2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked and nuclei were sonicated as described previously (Lu et al. 2015). Libraries were prepared via ChIPmentation (Schmidl et al. 2015).

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

244707593

Reads aligned (%)

62.2

Duplicates removed (%)

90.1

Number of peaks

1432 (qval < 1E-05)

hg19

Number of total reads

244707593

Reads aligned (%)

61.6

Duplicates removed (%)

91.1

Number of peaks

938 (qval < 1E-05)