Antigen

Antigen Class

TFs and others

Antigen

PHOX2B

Cell type

Cell type Class

Neural

Cell type

IMR-32

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

IMR32_PHOX2B_ChIPSeq

cell line

IMR32

cell type

Neuroblastoma

genotype/variation

siCtrl

chip antibody

PHOX2B (santa cruze, sc-376997 X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

29888344

Reads aligned (%)

91.0

Duplicates removed (%)

28.9

Number of peaks

2529 (qval < 1E-05)

hg19

Number of total reads

29888344

Reads aligned (%)

90.5

Duplicates removed (%)

30.0

Number of peaks

2462 (qval < 1E-05)